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The following conditions were used for the thermal cycler (T100 Thermal Cycler, BIO-RAD, Hercules, CA, USA): 10 min at 25 C, 120 min at 37 C, 5 min at 85 C, and 10 min at 4 C

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Lipo-B (MIC) Shot: Supports fat metabolism and may aid weight management as part of a healthy lifestyle

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