The cells treated for 1 h with 200 M of the ROS inducer TBHP were used as a positive control
Abnormalities of Homocysteine Metabolism Dysfunctions related to congenital defects in enzymes involved in homocysteine metabolism, to deficiencies in the supply, absorption, or metabolism of folates, vitamins B 12 and B 6 , and to dietary methionine excess all contribute to increasing plasma homocysteine levels (Table 1)
While BCL-2 protein expression upon CB-839 treatment remained stable even at maximal concentrations of 100 M, MCL-1 protein levels increased in both cell lines in cells incubated with CB-839 compared with control cells, indicating that the HG-3 and MEC-1 cells had an MCL-1 protein dependency in response to apoptotic stimuli ( Supplemental Figure 3 )
It is the body's primary intracellular antioxidant, operating in both the cytosol and mitochondria, and is directly responsible for regenerating Vitamin C, Vitamin E, and alpha lipoic acid
