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To remove any residual salts and ensure the ChIP DNA is as pure as possible, the DNA is washed with pre-cooled 70% ethanol, air-dried, and resuspended in double-distilled water or TE buffer (pH 8.0)

This simplifies cell culture applications and eliminates solvent-related confounding factors

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3xTg mice have been widely used to study geroprotective interventions, and CR has clearly beneficial effects on AD pathology and cognition in this model 4