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It was measured in human clinical trials using corneal confocal microscopy, a technique that directly visualizes and quantifies small nerve fiber density

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However, certain special situations, such as immature blood-brain barrier (BBB) in infants, compromised BBB, high-dose exposure, small molecular weight, few aromatic amino acids, light hydrophobicity, light cationic properties, transport mechanisms, and indirect effects, can affect BBB transitions (Domenger et al