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The second phase delved into the quantification of uncertainties arising from geometric and material variations

Glutathione is the liver's major antioxidant, often depleted in fatty liver disease due to oxidative stress and inflammation

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Therefore, noting the lack of spontaneous oxidation of free GSH between the two EDTA-treated samples stored at 80 o C, this result also suggests that the differences in GSH between the heparinized plasma (Preparation Condition 1) and deproteinized EDTA-treated plasma (Preparation Condition 2) are due to spontaneous oxidation of free GSH occurring in the heparinized plasma prior to the deproteinization process