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While several studies suggest that chronic circadian disruption from NSW may modestly elevate BC risk, findings have been heterogeneous and at times conflicting, reflecting variation in study design, exposure definitions, and adjustment for reproductive and lifestyle factors (Tables 1011)

doi:10.3390/nu11122985 [PubMed] 105

Research also suggests that a deficiency in lipotropes can lead to liver fat accumulation and other metabolic disturbances, highlighting their potential role in supporting liver function and metabolic balance

Real-time quantitative PCR Total RNA was extracted from aortic tissue and endothelial cells using TRIzol reagent (Takara Bio Inc., 9108), following the manufacturers instructions