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Percentage of cell viability was then calculated after subtracting background absorbance of cell culture medium 44

Step 1: Wash hands thoroughly with soap and water Step 2: Clean the vial stopper with an alcohol swab Step 3: Draw the appropriate dose into your syringe Step 4: Clean the injection site with alcohol and let it dry Step 5: Pinch skin to create a fold of subcutaneous tissue Step 6: Insert needle at 45-degree angle into the skin fold Step 7: Inject slowly over 5 to 10 seconds Step 8: Withdraw needle and apply light pressure if needed Local vs