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doi: 10.1073/pnas.1813458115 20 WangXSatoRBrownMSHuaXGoldsteinJL
The primary pathological hallmark of PD is the progressive loss of dopaminergic neurons in the substantia nigra pars compacta
Kit Components Additional Materials Required Microplate reader capable of measuring absorbance at 405 nm Precision pipettes to deliver 2 L to 1 mL volumes Multi-channel pipettes to deliver 20 L to 200 L volumes Tubes to prepare sample dilutions Ultrapure water 50 mL conical tubes Protocatechuic acid (HClO 4 ) (PCA) Potassium hydroxide (KOH) Assay Procedure Summary Add 50 L of the diluted GSSG Standards or unknown samples to the 96-well microtiter plate
The PRX family of proteins can be divided into three classes (typical 2-Cys, atypical 2-Cys [PRX5], and 1-Cys) with respect to the mechanism by which they decompose H 2 O 2 (Fig
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