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24 h post transfection, cells were fixed with 4% PFA for 10 min at RT, washed twice with PBS and permeabilized using a 0.1% NP-40 solution in PBS for 10 min at RT

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Because ipamorelin produces negligible cortisol and ACTH elevation, any observed downstream effects (IGF-1 changes, body composition shifts, sleep architecture changes) can be attributed to the GH axis rather than needing to be disentangled from concurrent adrenal activation

10.1016/S0006-2952(97)00401-2 Biochem