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Western blot Total proteins were extracted from cell lines or tissues, subjected to SDS-PAGE and analyzed by immunoblots as described previously [36, 37]

Nevertheless, administration of tissue plasminogen activator (tPA), the only approved thrombolytic agent, is constrained by its limited therapeutic window of three hours and by complications derived from hemorrhagic risks, reperfusion injury, and its own intrinsic toxicity (3)

Be sure to reduce fructose and oxalate levels during this period of time

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