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Arabidopsis putative selenium-binding protein 1 expression is tightly linked to cellular sulfur demand and can reduce sensitivity to stresses requiring glutathione for tolerance

[DOI] [PubMed] [Google Scholar] 117.Jandric I., Vrcic H., Jandric Balen M., Kolenc D., Brcic L., Radic B., Drmic D., Seiwerth S., Sikiric P

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Screening of GST fragments which can inhibit the GST activity 150 single positive clones (grown on the RGB plates), that could tightly bind to the glutathione Sepharose 4B, were picked up from the plates, and cultured separately in 50 ml of IMC medium containing 100 g/ml ampicillin for 15 hours at 25C, then induced with 100 g/ml tryptophan for 6 hours