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We invite public comments on whether the TOPS TM System meets the cost criterion and our proposal to approve new technology add-on payments for the TOPS TM System for FY 2024 subject to the technology receiving FDA marketing authorization as a Breakthrough Device for the indication corresponding to the Breakthrough Device designation by July 1, 2023

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Since it is suggested that p53, p16, p21 are directly related to the induction of senescence in chondrocytes (Ashraf et al., 2016), we thus used qRT-PCR and western blot analysis to examine their expression levels