immunocal platinum glutathione Immunotec Precursor Immunocal Supplement Pack of 2

TB-500, a synthetic fragment derived from thymosin beta-4, interacts with actin-associated cellular machinery that regulates cytoskeletal structure and cell migration

(2018) with slight modifications

It upregulates genes involved in collagen synthesis (COL1A1, COL3A1, COL5A1), extracellular matrix remodeling (decorin, fibromodulin), and antioxidant defense (superoxide dismutase, glutathione peroxidase) while simultaneously suppressing genes associated with inflammation (IL-6, TNF-alpha), fibrinogen production, and tissue destruction (several MMPs)

Flow cytometry Cells were collected, washed twice with PBS, and stained using 5 l of 7-AAD (BD Biosciences, #559925) and 5 l of annexin V-FITC (BD Biosciences, #556420) in 100 l of annexin V binding buffer (BD Biosciences, #556454) per sample