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CHIP is a chaperone-dependent E3 ligase that ubiquitylates unfolded protein

Mutating Phe260 to glycine (Phe260Gly) impaired both activities (monophenolase and diphenolase)

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Urine testing is one of the most widely used approaches because its inexpensive, straightforward, and reliable for spotting many commonly misused substances, but it has its limitations