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Cytosolic calcium concentration The concentration of cytosolic free Ca 2+ was determined by loading cells with the acetoxymethyl ester of the Ca 2+ -binding dye Fura-2 (Fura-2-AM, Molecular Probes, Sigma-Aldrich, USA) in the dark at 28 C for 1 h

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According to the OSIG letter, TRRC and Feinman violated TRRC policy in its dealings with Charles Lessin, who was, at that time, also CEO of Appalachian Biofuels, LLC

So, while they can help, they're not a long-term solution