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With 10 mL of water in a 2 mg vial, you might only get 4-5 usable doses before the liquid level drops too low to draw from effectively

Additionally, there was a notable reduction in oxidative stress markers

Rotational head kinematics in football impacts: An injury risk function for concussion

All gels were fixed for 1 hour in 10% v/v acetic acid and 40% v/v ethanol followed by Colloidal Coomassie blue staining (Phastgel Blue R, Amersham Biosciences) overnight