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An appropriate concentration of IPTG was added when the cell density reached the OD 600 value of 0.40.6

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A landmark 2021 study by researchers at Baylor College of Medicine investigated this phenomenon, finding that adults hospitalized with COVID-19 exhibited severe, systemic glutathione deficiency and exceptionally high levels of oxidative damage, regardless of their chronological age or prior health status

Moreover, in H4IIE cells, a rat hepatoma cell line, that were stably transfected with the cDNA for the 2-oxoglutarate carrier, exhibited increased uptake of GSH and 2-oxoglutarate and were protected from cytotoxicity induced by hydrogen peroxide, methyl vinyl ketone, or cisplatin, demonstrating the protective function of increased mitochondrial GSH transport and mGSH levels in the liver (153)