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Notably, most clinical investigations studied women (1404 female versus 83 male participants), which is expected since estrogen declines after age 30 years, and further estrogen decline with aging represents health challenges, as well as a reduction in QOL [9, 10, 19,20,21,22] and a degradation in the most visible organ of the bodythe skin [9, 10]

Following the peak laying period, late laying hens experience age-related oxidative stress that induces ovarian aging and endocrine hormonal imbalances, which are major contributors to the gradual decline in production performance and egg quality (39)

AP-1 and NF-B induce the expression of multiple genes involved in inflammation and oxidative stress response, cell death and fibrosis, including proinflammatory cytokines such as tumor necrosis factor- (TNF-), interleukin-1 and interleukin-6 and growth factors such as PDGF and TGF-