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The normal vascular endothelium regulates vasodilation and vasocontraction, proliferation and antiproliferation of vascular smooth muscle cells, coagulation and anticoagulation, inflammation and anti-inflammation, and oxidation and antioxidant effects, which work in balance to regulate and maintain vascular tone and structure [53]

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30 Briefly, the volunteers were habituated to test environment in a relaxed sitting position for 30 minutes to minimize inter-variability among the subjects due to tobacco intake prior to data acquisition