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Aliquots were centrifuged at 13,000 rpm for 10 min at 4 C, and 150 L supernatant was transferred to Chromacol vials containing 100 L water for dilution

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ROS are unstable derivatives of oxygen and can seriously damage living cells if formed in significant amounts (oxidative stress)

Target gene expression levels were normalized by 18 S ribosomal RNA (18 S ribosomal N1), and relative gene expression was calculated using the 2e(-Ct) method 21