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In the second scenario, the final yield of your elution is close to 100 mg of GST-fusion protein, so youre feeling confident that you used the right binding buffer and that your protein isnt too big

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doi: 10.3389/fnagi.2022.998292 Received 19 July 2022 Accepted 12 August 2022 Published 01 September 2022 Volume 14 - 2022 Edited by Zhigang Liu, Northwest A&F University, China Reviewed by Anubhuti Dixit, Amity University, India