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After RNA fragmentation, following the standard protocol of the Magna Methylated RNA Immunoprecipitation m6A Kit (Merck Millipore, Germany), immunoprecipitation was performed using m6A antibody

Mechanistic studies have revealed that altered expression of iron metabolism-related proteins (including GPX4, FTH1, TfR1, and HO-1), accumulation of lipid peroxidation products, and disruption of antioxidant defense systems (particularly the Nrf2/GPX4 pathway) are central to ferroptosis progression in cardiac tissue

In both ways, we observed a neuroprotective effect in our model