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Cell culture, treatment, and transfection Human HEK293T and HT1080 cells were obtained from the American Type Culture Collection (ATCC) and maintained (37 C, 5% CO 2 ) in DMEM high-glucose medium (Sigma-Aldrich), supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich), penicillinstreptomycin (100 U/mL, Gibco, 15140148), sodium pyruvate (1 mM, Gibco, 11360070), and MEM nonessential amino acids (1, Gibco, 11140050)

Studies suggest this derivative may address both extrinsic photoaging and intrinsic hyperpigmentation without the formulation challenges of parent compound ascorbic acid

L-Glutathione, or simply glutathione, is a powerful antioxidant comprised of amino acids cysteine, glycine, and glutamic acid

11 647 229 001) or the CellTiter-Glo 2.0 Cell Viability Assay from Promega (G9241)