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At 72 h after seeding medium was replaced for DMEM without FBS containing test compound

Western blot Cell or tissue lysates (20 g) were denatured, separated by SDS-PAGE, and transferred to polyvinylidene difluoride (PVDF) membranes

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Additionally, it was demonstrated, that failure PINK/PARKIN axis expression favors the accumulation of defective mitochondria that are not removed from the cells due to the impaired lysosomal system which in consequence promotes cellular damage, generates oxidative stress, and impairs sensitivity for insulin [60]