Proceedings of the National Academy of Sciences of the United States of America, 97 (10), 5071-5076
Bone-marrow cells were stained with the antibody mixture (lineage cocktail (Mac1 (CD11b), Gr1 (Ly-6G/C), Ter119 (Ly-76), CD3, CD4, CD8a (Ly-2), and B220 (CD45R)), c-Kit, Sca1, CD48 and CD150 antibodies) (BioLegend, 101226, 108424, 116223, 100222, 100414, 100714, 103224, 105812, 108120, 103404 and 115904) for 20 min at 4 C, washed and analyzed by flow cytometry
Beyond-use dating principles from the United States Pharmacopeia General Chapter treat preservative-containing solutions as having longer safe in-use windows than preservative-free ones
If you have Wilson's disease, are pregnant or breastfeeding, have active cancer, or have demonstrated copper allergy, avoidance is the prudent choice