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Conversely, [U- 13 C]glucose tracing showed GGCT overexpression enhanced Glc-derived carbon flux into the TCA cycle (increased m + 2 labeling of succinate, fumarate, malate), which was reversed by the E98A mutant (Fig

Mixing too much at once increases the risk of the compound degrading before you can use it all

From that point, degradation begins, slowly at first but accelerating over time