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71,72,73,76 One obstacle to preclinical use in animal models is the species specificity of the drug

It appears that once steatosis is established, further NAFLD/NASH progression does not promote additional alterations in diacylglyceride-linked lipid metabolism, rendering diacylglycerides to exert an adverse effect already during the early stages of NAFLD development

TLK199 has also been shown to be an inhibitor of the multidrug resistance-associated protein-1 (MRP-1), reversing the resistance to a variety of agents in NIH3T3 cells transfected with MRP-1 (OBrien et al ., 1999)