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The progression from inflammation to fibrosis is primarily mediated by hepatic stellate cell (HSC) activation, which involves two core mechanisms: the Transforming Growth Factor-beta 1 (TGF-1) - Mothers Against Decapentaplegic Homolog 3 (Smad3) pathway and Platelet-Derived Growth Factor (PDGF) -driven epithelial-mesenchymal transition (EMT) 52 , Furthermore, Wnt ligands released by apoptotic bodies promote HSC proliferation through -catenin nuclear translocation 53
The cell pellets were cultured in chondrogenic medium (low-glucose Dulbeccos modified Eagle medium containing 0.1 mmol/L ascorbic acid 2-phosphate, 100 nmol dexamethasone, 40 g/mL proline, 100 U/mL penicillin, 100 g/mL streptomycin, and ITS Premix
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