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Through several mechanisms, including direct interaction with SUR1 and membrane depolarization, intracellular calcium mobilization, and insulin granule priming modification, EPAC2 activation may promote the exocytosis of insulin granules [55, 56]

diluted 1:10 in nuclease-free water) and 24 l Lipofectamine TM RNAiMAX (10514953, Thermo Fisher Scientific) were diluted separately in 1.5 ml Opti-MEM TM Medium (10149832, Thermo Fisher Scientific)

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