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Temporary Darkening of Freckles: For laser and IPL treatments, the treated freckles will initially darken significantly (like coffee grounds or pepper specks) before flaking off

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For quantification, 10 L of the hexane extract from each sample and standards (lupeol and -sitosterol) were applied as bands (8 mm) using Linomat V sample applicator and 100 L CAMAG (Muttenz, Switzerland) syringe onto silica gel G60 F 254 TLC plates (0.2 mm thick