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Chromatins were sonicated to 2001000 bp and immunoprecipitated with antibodies against NRF2, H3K4Me3, and H3K9Ac and determined with gene-specific primers (Supplementary Table 1)

Due to its fast reaction rate, the reaction between Ellman's reagent and thiol groups is widely used to measure thiol concentrations in biological and chemical systems

doi: 10.1111/nph.19081, 40

Moreover, current literature suggests that gut microbiome is a strong contender in metformin therapy and the microbiome-regulated mechanisms involving alterations in the gut microbiota composition influence its pleiotropic effects (Pryor et al., 2019