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[DOI] [PubMed] [Google Scholar] 18.Panizo S., Martnez-Arias L., Alonso-Montes C., Cannata P., Martn-Carro B., Fernndez-Martn J.L., Naves-Daz M., Carrillo-Lpez N., Cannata-Anda J.B

For example, a study of MTHFR polymorphism genotypes in colorectal cancer patients reported that the frequency of methylated Bcl-2 promoter was significantly higher in individuals with CC genotype than that of those with CT and TT genotypes, and a significant difference of C and T alleles distribution were observed between patients with methylated and unmethylated Bcl-2 promoter 35

Cells were then treated (duplicate wells/treatment) with stuarosporine (1 M) for 24 h [21] or H 2 O 2 (1002500 M, Sigma-Aldrich) for 2 h at 37 C a 30 min pre-treatment with inhibitors of cell death: liproxstatin (LPX, 10 M, Sigma-Aldrich) and necrostatin-1 (25 M) [22]