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The fractions containing OsDHAR protein were pooled and concentrated using Amicon Ultra-15 Centrifugal Filters (Ultracel-10 K

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As a result, the overexpression of RIP1, RIP3, and MLKL in the entire epidermis layers was dramatically blocked by EGCG-NPs, as well as the expression ofphosphorylated p38 (p-p38), extracellular signal-regulated kinase 1 (ERK1), and extracellular signal-regulated kinase 2 (ERK2)

Nociceptin/Orphanin FQ Nociceptin/orphanin FQ (N/OFQ) is peptide (IUPHAR/BPS, 2020), classified as a non-classical or non-opioid member (Singh et al., 2016)