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For differentiation of mouse SVF cells, the cells were first cultured to confluence in DMEM/F12 medium supplemented with 10% FBS and 1% P/S, induced to differentiation in the adipogenic cocktail consisting 1 M dexamethasone, 5 g/ml Insulin, 0.25 mM IBMX, 5 M rosiglitazone, followed by maintenance treatment with 1 g/ml Insulin and 5 M Rosiglitazone every 48 h until fully differentiated
There is no recommendation to use this treatment for an extended period of time
As a consequence of this gonadotropin stimulation, nuclear thyroid receptors in the granulosa cells are activated leading to ovarian hyperstimulation
Also, IF decrease plasma levels of ceramide C17, C22, and C24 sphingomyelin, as well as C20, C22, C24, and C24:1 dihydrosphingomyelin ( coactivator 1 (PGC-1), a transcription factor of mitochondria function, in neurons (Wilhelmi de Toledo et al., 2020
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