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The dialyzed proteins were concentrated using the 3 K centricon (Millipore, Sigma) and either proceeded fresh for the droplet formation assays or aliquoted and flash frozen in liquid nitrogen and stored at 80 C

Similar content being viewed by others Introduction Constipation is a prevailing condition featured by difficulties in defecation, including infrequent bowel movements, hard or lumpy stools, excessive straining, sensation of incomplete evacuation or blockage, and use of manual maneuvers to facilitate evacuation [1]

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GST was able to convert the derivative into the parent drug in a quantitative manner (Fig