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Superparamagnetic iron oxide nanoparticles (SPIONs), which contain 5-Flurouracil as a chemotherapeutic medication, were created by Raviraj et al

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2.5.2 MDA content 5 mL 10% trichloroacetic acid solution was added to a 1 g tissue sample and centrifuged at 10,000 g for 25 min to obtain the supernatant

In summary, nanoparticles can not only exert antitumor activity by regulating TAMs, NK cells, DCs, TANs, MDSCs, and T cells in the TME, but also simultaneously regulate two or more immune cells to exert antitumor immunity, such as simultaneously regulating NK cells and T cells, regulating TAMs and T cells, regulating DCs and T cells, as well as nanoparticles that simultaneously regulate multiple immune cells