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NNMT enzyme inhibition occurs within 24 hours of subcutaneous dosing based on pharmacokinetic modelling, but downstream metabolic effects NAD+ elevation, changes in fat oxidation markers typically require 24 weeks of consistent dosing to manifest in measurable endpoints

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Proximity ligation assay (PLA) for Cntfr and Syt1 Labelling of Cntfr was performed as described above also including mouse anti-Syt1 luminal antibodies (1:150 dilution, Synaptic Systems, cat