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Synthesis of cDNA was done by using the iScript TM cDNA synthesis kit (Bio-rad, Hercules, USA) as described by the manufacturer

Endocr Pract: Off J Am Coll Endocrinol Am Assoc Clin Endocrinol

All lenses were incubated for 3 to 4 days in 2 mL of M199 medium (Sigma-Aldrich) containing 0.1% BSA and 30 mM Galactose using an incubator set at 5% CO 2 and 37 C to induce opacity, as previously reported 30

Supplementation or approaches that increase endogenous synthesis, such as N-acetylcysteine, glycine, and cysteine availability, demonstrate measurable improvements in oxidative stress markers and, in some studies, improved fertilization outcomes