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Lower GSH levels and GSH to oxidized GSH ratio (GSH:GSSG), as measures of redox balance, have previously been reported in asthmatic lungs (3), although the external or internal immunoinflammatory pathways driving this dysregulation are unknown

Superoxide measurement Cells were grown in a 12 well plate to about 50% confluence after which the media were removed and the cells were washed with HBSS (Hanks Balanced Salt Solution) followed by a 1 h pretreatment with 25 M dihydroethidium (DHE), from Life Technologies

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