Most of these proteins act as chemotactic factors at the site of inflammation, promoting the initial recruitment of satellite cells, neutrophils, monocytes, and, later, lymphocytes for tissue repair [52, 62,63,64,65]
Recently, a newly founded bifunctional enzyme GshF from Actinobacillus succinogenes had be discovered in our lab
tumefaciens strain GV3101 carrying pYL156-RNA1 , pYL156-MEKK1 , pYL156-BAK1 / SERK4 , pYL156-CLA1 or pYL156-GFP (the vector control) was grown in LuriaBertani (LB) liquid medium containing 50 g ml 1 kanamycin and 25 g ml 1 gentamicin at 28 C for 48 h, and the culture was then transferred into fresh liquid LB medium containing 50 g ml 1 kanamycin, 25 g ml 1 gentamicin, 10 mM MES and 20 M acetosyringone for overnight incubation at 200 rpm at 28 C
doi: 10.1016/j.pnpbp.2006.10.001