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A prospective clinical study has demonstrated that ferroptosis levels showed considerable promise as a novel monitoring indicator and therapeutic target, as they play a critical role in predicting the healing rate of DFUs and assisting clinical treatment decisions [24]

After washing with DMSO and Milli-Q (Millipore, Billerica, MA, USA) water, the tips were incubated with 10 L of a 10 M solution of p29 in 50 mM phosphate buffered saline (PBS), pH 7.5, overnight at 4C

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The two main approaches are subcutaneous injection (under the skin, typically in the abdominal fat pad) and intramuscular injection (into a muscle belly)