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The dissociation of the complexes leads to ASK1 activation and prolonged activation of P38 and JNK that cause cell growth arrest by increasing the expression of the CDK inhibitor P21 CIP1 and apoptosis

Epub 2012 Mar 9

The resulting supernatant was used to determine the adrenal catalase, MDA, total protein, reduced glutathione and nitric oxide

Proteoliposome counterflow assays Frozen proteoliposome and protein-free liposome aliquots (lipid concentration: 8 mg/ml) were thawed and diluted twofold in internal liposome buffer with final concentrations of 150mM KCl, 50mM HEPES (pH 7.4), 5 mM (Figure 3a and Extended Data Figure 4l) or 10 mM (Extended Data Figure 7F) unlabeled GSSG (Thermo J63715.06)