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Description

The NAD + -consuming enzymes produce NAM as a byproduct, of which high concentrations inhibit the activity of NAD + -consuming enzymes, providing feedback regulation

Cells were seeded in 8-well LabTek removable chamber slides (Thermo Scientific, 177402) to 60%70% of confluence

Wakefulness time measurement To evaluate the impact of Pichia pastoris secreted recombinant peptides on insomnia, we analyzed the increase in wakefulness time among different groups of mice: Control, Model, GABA, DSIP-CBBBP, DSIP, and CBBBP