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Gay CM, Diao L, Ca S, Zi Y, Cardnell RJ, Swisher SG , Roth JA, Glisson B, Wang J, Heymach JV, Byers LA

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subsequent research has documented similar effects for cannabis, gambling, and trichotillomania all compulsive behavior patterns with glutamatergic underpinnings Bipolar depression: Berk et al

To obtain lentivirus, the pCDH-ECM1-HA or pCDH or pLKO.1-ECM1 or pLKO.1 or pCDH-ECM1- 1(amino acids 1-177)-HA or pCDH-ECM1-2(amino acids 178432)-HA or pCDH-ECM1-3(amino acids 433540)-HA or sgRNA constructs were co-transfected with packaging plasmids pSPAX2 and pMD2G into 80% confluent HEK293T cells