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To exclude the possibility that other lysine acetylation sites in SnCE1 K231R or SnCE1 E217D K231R might prohibit tetramer formation, we deacetylated SnCE1 K231R and SnCE1 E217D K231R by SIRT1 and performed analytical SEC

This is generally not a concern for oral or liposomal formats

Endocrinology 156(1):255267 Hamilton A, Holscher C (2009) Receptors for the insulin-like peptide GLP-1 are expressed on neurons in the CNS Neuroreport 20: 1161 Dupont J, Khan J, Qu BH, Metzler P, Helman L, LeRoith D (2001) Insulin and IGF-1 induce different patterns of gene expression in mouse fibroblast NIH-3T3 cells: identification by cDNA microarray analysis