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The remaining colon samples were stored at 80C for future protein blot analysis

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To verify whether HOXA2 was regulated by DNA methylation in TGF-1-stimulated HK-2 cells, the activity of DNMT1 was tested, and the results revealed that DNMT activity was upregulated significantly after TGF-1 treatment and was effectively inhibited by the DNA methylation transferase inhibitor drug 5-Aza in HK-2 cells (Fig

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