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To accurately assess neuron density, five non-overlapping fields of view within each brain tissue section were selected to represent the overall tissue morphology

Subsequently, the plate was incubated in the Cytation3 (BioTek, Winooski, VT, United States) at 37 C with 5% (v/v) CO 2 for 30 min

This mitochondrial failure is the direct biochemical driver of the profound, debilitating fatigue and post-exertional malaise (PEM) that patients experience after even minor physical or cognitive exertion

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