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They also think that we all need the same measly amount regardless of our symptoms and level of damage

Reverse dimerization assay HeLa cells were co-transfected with the Golgi marker GalT-mRFP (not displayed in all figures) and Go-Nt 7 -FM 4 -GFP, MGNC-Nt 7 -FM 4 -GFP or GFP-FM 4 -hGH (at a 1:3 plasmid ratio), and seeded on poly-L-lysine-coated coverslips for 15 h as indicated above

Binding of GH to GHBP prolongs the circulation time of GH as the complex is not filtered by the glomeruli (300)

The concentration of Human REN in the samples is then determined by comparing the OD of the samples to the standard curve