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Model 1 As a first step, the following general linear model was used to test the association between smoking status category (that is, smokers, ex-smokers and those who have never smoked) and cortical thickness at each vertex: Cortical thickness= b 0 Intercept+ b 1 Gender+ b 2 Exact_Age_At_Scanning+ b 3 Smoking-Category+error Model 2 Next, in order to more effectively assess the doseresponse relationship between smoking and cortical thickness, the Smoking-Category term was replaced by a Pack-Years term