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False-positive selections and contaminants were excluded through iterative rounds of heterogeneous classification using three references and four decoy classes generated from noise particles through ab initio reconstruction in cryoSPARC (v4.5.0), resulting in stacks of 877,231, 815,775, and 906,048 particles from each dataset

Approximately 80% of SURF1 mutations are truncating, caused by abnormal splicing, frameshift deletions, or nonsense mutations

After injection, apply pressure on the site where you injected B12

Why it matters: Ensures the peptide remains structurally intact and stable during shipping and long-term storage